Vertical Distribution of Shellfish Larvae: Metagenomic Analysis of Autonomously-collected Larvae

We will study the vertical distribution of sea scallop, ocean quahog, and surf clam larvae in ocean layers; implement newly developed autonomous sampling methods for improved spatial resolution; and develop ìnext generationî genetic sequencing methods to identify the larvae. An AUV REMUS 600 will be equipped with a Suspended Particle Rosette (SUPR) Sampler to collect larvae on three dates in late summer/early fall, along with a CTD to measure and characterize the vertical structure of the water column. Larvae samples will be identified using conventional and next-generation DNA barcoding. Our results will provide information for commercially valuable bivalve species that will be made available directly to scientists developing meta-population and numerical dispersal models.