Publication Detail

A colorimetric method for the enzymatic analysis of gases: the determination of ethanol and formaldehyde vapors using solid alcohol oxidase.

Eduardo Barzana Garcia, Marcus Karel, Alexander M Klibanov
MITSG 89-18P

A novel approach to direct determination of ethanol vapors in the gas phase uses dehydrated enzymes and an indicator dye dispersed in avicel. Simple devices are developed for determination of ethanol in the breath. The devices produce a sharp color change at a set time for ethanol concentrations above the legal limit for driving or a stable final color after five minutes. The system can also be used for determination of ethanol or formaldehyde vapors. In addition to determination of ethanol, dehydreated enzymes may be useful for analysis of hazardous gases.

type: Technical reports

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