Publication Detail

Effect of pH on Lipid Oxidation Using Trout Hemolysate as a Catalyst: A Possible Role for Deoxyhemoglobin

Mark P. Richards, Herbert Hultin
2000
7 pp.
MITSG 00-03J

A quasi-lipoxygenase activity of hemoglobin has been described. If hemoglobin acts as an enzyme, changes in the electrostatic charge of amino acids due to pH adjustment may affect catalysis of lipid peroxidation oat the active site. The main objective of this work was to investigate the effect of pH on lipid oxidation in washed minced cod muscle caused by trout blood hemolysate as a source of hemoglobin. Three pH values were examined. The lag time prior to rancidity and thiobarbituric acid reactive substance development decreased greatly as the pH was reduced. Formation of methemoglobin due to autoxidation of the heme pigment was found to occur more rapidly at reduced pH. These and other findings suggest that reduced hemoglobins played an important role in lipid oxidation processes.

type: Technical reports

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Parent Project

Project No.: 2000-RB-50
Title: Production of High Value Food: Proteins from Low Value, Underutilized Fish

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